Figure 4.
Accelerated Global Gene Expression Changes toward iPSCs by Smad3CA
(A) Global gene expression comparison between reprogramming with control (Cont) and +Smad3CA (+Sm3CA) vector expression on days 3, 6, 8, and 10. The diagonal dashed lines represent 2-fold differences.
(B) Gene set enrichment analysis (GSEA) for the pluripotency genes highlighted in (A). ES, enrichment score.
(C) Genes >4-fold up- and downregulated in ESCs compared to MEFs. Solid and dotted diagonal lines represent 1-fold and 4-fold changes, respectively. The x and y axes show log2 read counts.
(D) Read count ratios (+Sm3CA/Cont) of all 18,909 genes, 438 ESC upregulated genes, and 815 ESC downregulated genes at days 3, 6, 8, and 10 are shown with boxplots. Red crosses represent mean.
(E) Scatterplots (+Sm3CA versus Cont) of ESC up- and downregulated genes in (C) and (D). Solid and dotted diagonal lines represent 1-fold and 2-fold changes, respectively.