Figure 6.

Smad2/3CA Potentiate Macrophage and Adipocyte Conversions

(A) Conversion of B cells to macrophages by retroviral transduction of Cebpα with or without Smad2CA (Sm2CA) or Smad3CA (Sm3CA).

(B) Expression changes of the B cell marker CD19 and the macrophage marker MAC-1 during the Cebpα-mediated transdifferentiation process with additional control (Cont), Sm2CA, or Sm3CA vector expression.

(C) Quantification of CD19 and MAC-1 expression.

(D) Changes in forward scatter (FSC) and side scatter (SSC).

(E) qPCR analysis of macrophage and pre-B cell markers.

Graphs in (C) and (E) represent averages of 2 independent experiments with 2 technical replicates, respectively. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001 (two-sided t test).

(F) Conversion of C2C12 myoblasts to adipocytes with expression of Cebpβ and Prdm16 with or without Sm2CA or Sm3CA.

(G) Oil red O staining of cells 6 days post induction of Cebpβ and Prdm16 with an additional control (Cont), Sm2CA, or Sm3CA vector.

(H) Quantification of oil red O staining. Graphs represent averages of 2 independent experiments, with 3 technical replicates, one of those images is presented in G. ^∗∗p < 0.01; ^∗p < 0.05 (two-sided t test).

See also Figure S5.