Fig. 1.

PLK1 knock-down hinders cell proliferation, invasion and migration. The mRNA (a) and protein expression (b, c) levels of PLK1 were examined by qPCR and western blotting. S: SV-HUC-1, R: RT4, B: BIU-87, 5: 5637, T: T24. The efficiency of PLK1 knockdown by siRNA was determined by western blotting (d, e). The cell proliferation was examined by the MTT assay (f) and BrdU assays (g) in control siRNA groups and the PLK1 siRNA group. The transwell assay was used to examine the cell migration (h) and (i) invasion in control siRNA group and PLK1 siRNA groups