Fig. 4.

Validation ofe the five representative key genes regulated by PLK1 in bladder cancer cells. The mRNA (a) and protein expression (b, c) levels of five genes (BUB1B, CCNB1, CDC25A, FBXO5, NDC80) were examined by qPCR and western blotting. SV: SV-HUC-1, T24: T24. The mRNA (d) and protein expression (e, f) levels of the five genes were determined in T24 cells with PLK1 knockdown by qPCR and western blotting. The Cell proliferations were abilities were examined by the MTT assay (g) in the five genes specific siRNA groups. The transwell assay was used to examine the cell migration (h) and (i) invasion in the five genes gene-specific siRNA groups