Table 3.
Candidate effectors for avirulence genes in Puccinia striiformis f. sp. tritici.
| Protein ID | Length (aa) | Cysteine | Haustorial differentially expressed | Subcellular location | Homology to (e-value) | PFAM domain | Note |
|---|---|---|---|---|---|---|---|
| AvYr6 CANDIDATES | |||||||
| PSTG_00485 | 130 | 4 | No | Secreted | – | Lytic transglycolase (PF03330) | – |
| PSTG_02758 | 190 | 8 | No | Secreted | – | – | Motif [Y/F/W]XC |
| PSTG_06067 | 368 | 2 | Yes | Nucleus | – | – | – |
| PSTG_06250 | 204 | 4 | No | Vacuole | – | ML domain (PF02221) | Pst specific |
| PSTG_07109 | 191 | 7 | Yes | Secreted | – | – | – |
| PSTG_08586 | 86 | 7 | No | Secreted | – | – | Pst specific |
| PSTG_09464 | 279 | 4 | No | Mitochondrion | Zymoseptoria tritici ZtCBR1 (2.02e-82) | Oxidoreductase FAD-binding domain (PF00970,PF00175) | Reduced virulence in Z. tritici (Derbyshire et al., 2015) |
| PSTG_10570 | 120 | 6 | No | Secreted | – | – | Pst specific |
| PSTG_12812 | 125 | 4 | No | Secreted | – | – | Motif RSIVEQD |
| PSTG_12835 | 265 | 5 | Yes | Secreted | – | Lipase (class 3) (PF01764) | – |
| PSTG_12889 | 259 | 10 | No | Secreted | – | – | – |
| PSTG_13579 | 143 | 9 | No | Secreted | – | – | Pst specific |
| PSTG_14087 | 293 | 10 | Yes | Secreted | – | – | – |
| PSTG_15607 | 220 | 5 | Yes | Secreted | – | – | – |
| PSTG_15157 | 102 | 6 | No | Secreted | – | – | Pst specific |
| PSTG_15482 | 152 | 8 | No | Secreted | – | – | – |
| PSTG_16061 | 232 | 7 | Yes | Secreted | – | CFEM domain (PF05730) | Fungal pathogenesis (Kulkarni et al., 2003) |
| AvYr8 CANDIDATES | |||||||
| PSTG_08473 | 215 | 6 | No | Plasma membrane | Xanthomonas campestris pv vesicatoria CaRING1 (2.74e-6) | Ring finger domain (PF13639) | Required for cell death and the salicylic acid-dependent defense response (Lee et al., 2011) |
| PSTG_12383 | 446 | 5 | Yes | Secreted | Verticillium fungicola vfglu1 (3.67e-19) | Cellulase (glycosyl hydrolase family 5) (PF00150) | Reduced virulence in V. fungicola |
| AvYr9 CANDIDATES | |||||||
| PSTG_05108 | 546 | 7 | Yes | Nucleus | – | – | Pst specific |
| PSTG_08524 | 185 | 1 | Yes | Nucleus | – | – | Motif RXLR |
| PSTG_12319 | 111 | 7 | Yes | Secreted | – | – | Pst specific |
| PSTG_14207 | 238 | 4 | Yes | Mitochondrion | – | – | – |
| PSTG_16854 | 674 | 8 | No | Nucleus | Ustilago maydis fuz7 (5.08e-156) | Protein kinase domain (PF00069) | Virulence factor in U. maydis (Lanver et al., 2010) |
| AvYr76 CANDIDATE | |||||||
| PSTG_18147 | 102 | 8 | No | Secreted | – | – | Pst specific; Gene presence/absence polymorphism |
| CANDIDATES SHOWING HIGH SIMILARITY TO PHI DATABASE | |||||||
| PSTG_09480 | 298 | 7 | No | Secreted | Phytophthora_sojae XEG1 (9.27e-14) | Glycosyl hydrolase family 12 (PF01670) | Plant avirulence determinant in P. sojae (Ma et al., 2015) |
| PSTG_09484 | 294 | 6 | No | Secreted | Phytophthora_sojae XEG1 (4.75e-16) | Glycosyl hydrolase family 12 (PF01670) | Plant avirulence determinant in P. sojae (Ma et al., 2015) |
| PSTG_10154 | 266 | 11 | Yes | Secreted | Magnaporthe oryzae ACE1 (7.26e-3) | – | Plant avirulence determinant in M. oryzae (Böhnert et al., 2004) |
| PSTG_02725 | 242 | 11 | Yes | Endoplasmic reticulum | Xanthomonas campestris pv vesicatoria CaRING1 (4.96e-5) | Ring finger domain (PF13639) | Required for cell death and the salicylic acid-dependent defense response (Lee et al., 2011) |
| PSTG_12818 | 278 | 14 | No | Endoplasmic reticulum | Xanthomonas campestris pv vesicatoria CaRING1 (4.88e-5) | Ring finger domain (PF13639) | Required for cell death and the salicylic acid-dependent defense response (Lee et al., 2011) |
| PSTG_14686 | 228 | 7 | Yes | Plasma membrane | Xanthomonas campestris pv vesicatoria CaRING1 (6.25e-5) | Ring finger domain (PF13639) | Required for cell death and the salicylic acid-dependent defense response (Lee et al., 2011) |
| PSTG_15141 | 250 | 12 | Yes | Plasma membrane | Xanthomonas campestris pv vesicatoria CaRING1 (2.32e-4) | Ring finger domain (PF13639) | Required for cell death and the salicylic acid-dependent defense response (Lee et al., 2011) |
| PSTG_01920 | 204 | 4 | No | Cytoplasm | Candida_albicans SOD5 (1.76e-18) | Copper/zinc superoxide dismutase (PF00080) | Loss of pathogenicity in C. albicans (Martchenko et al., 2004) |
| PSTG_04011 | 343 | 5 | No | Peroxisome | Candida_albicans SOD5 (1.02e-13) | Copper/zinc superoxide dismutase (PF00080) | Loss of pathogenicity in C. albicans (Martchenko et al., 2004) |
| PSTG_07834 | 196 | 8 | No | Cytoplasm | Candida_albicans SOD5 (1.02e-13) | Copper/zinc superoxide dismutase (PF00080) | Loss of pathogenicity in C. albicans (Martchenko et al., 2004) |
| PSTG_10359 | 210 | 6 | Yes | Peroxisome | Candida_albicans SOD5 (1.47e-7) | Copper/zinc superoxide dismutase (PF00080) | Loss of pathogenicity in C. albicans (Martchenko et al., 2004) |
| PSTG_10364 | 178 | 7 | Yes | Cytoplasm | Candida_albicans SOD5 (1.92e-8) | Copper/zinc superoxide dismutase (PF00080) | Loss of pathogenicity in C. albicans (Martchenko et al., 2004) |
| PSTG_11365 | 176 | 7 | Yes | Cytoplasm | Candida_albicans SOD5 (3.52e-7) | Copper/zinc superoxide dismutase (PF00080) | Loss of pathogenicity in C. albicans (Martchenko et al., 2004) |
| PSTG_11369 | 186 | 8 | No | Cytoplasm | Candida_albicans SOD5 (2.55e-12) | Copper/zinc superoxide dismutase (PF00080) | Loss of pathogenicity in C. albicans (Martchenko et al., 2004; Gleason et al., 2014) |
| PSTG_15532 | 179 | 5 | No | Cytoplasm | Candida_albicans SOD5 (8.44e-11) | Copper/zinc superoxide dismutase (PF00080) | Superoxide dismutase in C. albicans (Martchenko et al., 2004) |
| PSTG_04010 | 187 | 5 | No | Cytoplasm | Candida_albicans SOD5 (1.24e-11) | Copper/zinc superoxide dismutase (PF00080) | Superoxide dismutase in C. albicans (Martchenko et al., 2004) |
| PSTG_09596 | 204 | 5 | No | Secreted | Candida_albicans SOD5 (6.51e-6) | Copper/zinc superoxide dismutase (PF00080) | Superoxide dismutase in C. albicans (Martchenko et al., 2004) |
| PSTG_10366 | 193 | 4 | No | Secreted | Candida_albicans SOD5 (1.54e-5) | Copper/zinc superoxide dismutase (PF00080) | Superoxide dismutase in C. albicans (Martchenko et al., 2004) |
| PSTG_11367 | 192 | 4 | No | Mitochondrion | Candida_albicans SOD5 (1.08e-5) | Copper/zinc superoxide dismutase (PF00080) | Superoxide dismutase in C. albicans (Martchenko et al., 2004) |
| PSTG_11370 | 186 | 4 | No | Cytoplasm | Candida_albicans SOD5 (1.04e-11) | Copper/zinc superoxide dismutase (PF00080) | Superoxide dismutase in C. albicans (Martchenko et al., 2004) |
| PSTG_02563 | 159 | 3 | Yes | Cytoplasm | Botrytis cinerea BcFKBP12 (6.80e-19) | FKBP-type peptidyl-prolyl cis-trans isomerase (PF00254) | Sulfur regulation and pathogenic development in B. cinerea (Meléndez et al., 2009) |
| CANDIDATES WITH KNOWN EFFECTOR MOTIFS | |||||||
| PSTG_00821 | 108 | 7 | Yes | Secreted | – | – | Motif [Y/F/W]XC; Pst specific |
| PSTG_03385 | 128 | 7 | Yes | Secreted | – | – | Motif [Y/F/W]XC |
| PSTG_05233 | 126 | 6 | Yes | Secreted | – | – | Motif [Y/F/W]XC; Pst specific |
| PSTG_06302 | 118 | 6 | Yes | Secreted | – | – | Motif [Y/F/W]XC |
| PSTG_08956 | 127 | 6 | Yes | Secreted | – | – | Motif [Y/F/W]XC |
| PSTG_11601 | 98 | 7 | Yes | Secreted | – | – | Motif [Y/F/W]XC; Pst specific |
| PSTG_14695 | 152 | 6 | Yes | Secreted | – | – | Motif [Y/F/W]XC |
| PSTG_19156 | 111 | 7 | Yes | Secreted | – | – | Motif [Y/F/W]XC; Pst specific |
| PSTG_19591 | 68 | 4 | Yes | Secreted | – | – | Motif [Y/F/W]XC; Pst specific |