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. 2017 Dec 16;7:71. doi: 10.1186/s13578-017-0197-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Bar graphs represent relative band intensity of map2k1 and eif4b. Quantified mean protein value is relative to Ponceau which was used for normalization. To account for the difference in protein loading during the experiment, the percentage of regulation was calculated after the intensity of each band was adjusted according to its respective Ponceau band intensity using the ImageJ 2.1.4.7. software (National Institutes of Health). Representative western blots are depicted, showing map2k1 and eif4b expression in HKCSCs untreated, resistant and sensitized cells in normoxia and hypoxia. Error bars presented as mean ± SD