Table 1.
Mass spectrometry analysis results of chosen proteins
| Protein name | Gene name | Full name | qvalue | Ratio | Fold change | Peptide no. | Citation (protein association with RCC or cancerogenesis or hypoxia) | Function (main process) |
|---|---|---|---|---|---|---|---|---|
| P23588 | eif4b | Eukaryotic translation initiation factor B | NA | SH/AN: only rSH N: 0 rAN: 0 H: 0 rSH: 1 |
NA | 2 | [57] | Signal transduction |
| Q02750 | map2k1 | Dual specificity mitogen-activated protein kinase 1 | NA | N/H: only H N: 0 H: 1 rSH: 0 rAN: 0 |
NA | 5 | [58, 59] | Signal transduction |
| Q99459 | cdc5 l | Cell division cycle 5-like protein | NA | Only rSH (rSH/rAN) | NA | 2 | [60] | Cell cycling |
| O60264 | smarca5 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 | NA | Only rSH (rSH/rAN) | NA | 3 | [61] | Nucleosome-remodeling activity |
| Q9UIG0 | baz1b | Tyrosine-protein kinase BAZ1B | NA | Only rAN (AN/N) | NNA | 2 | [62] | Chromatin remodeling |
| P07910 | hnrnp | Heterogene-ous nuclear ribonucleo-proteins C1/C2 | NA | Only rAN (rAN/N) | NNA | 2 | [63] | Regulation of mRNA splicing |
| P14618 | pkm | Pyruvate kinase PKM | 0.00013 (H/N) 0.00011 (rSH/rAN) |
2.34 (H/N) 1.89 (rSH/rAN) |
2.34 (H/N) 1.89 (rSH/rAN) |
45 (H/N) 42 (rSH/rAN) |
[64] | Metabolic (production of ATP) |
| P05091 | aldh2 | Aldehyde dehydroge-nase, mitochon-drial | NA | Only H (H/N) Only rAN (rAN/N) |
NA | 4 (H/N) 3 (rAN/N) |
[65] | Alcohol metabolism, marker for stem cells |
| Q9BXP5 | srrt | Serrate RNA effector molecule homolog | NA | Only H (SH/H) | NA | 2 | [66] | Cell proliferation |
| P62258 | 14-3-3ε | 14-3-3 epsilon | 0.00027 | 1,7 (rSH/rAN) | 1,7 (rSH/rAN) | 21 | [67] | Is believed to play a role in G2/M transition |
| Q9NYF8 | bclaf1 | Bcl-2-associated transcription factor 1 | NA | Only rSH (rSH/H) | NA | 2 | [68] | Apoptosis, negative regulation of transciption |
| P00338 | ldha | L-lactate dehydrogenase A chain | 0.00011 (SH/AN) 0.00816 (H/N) |
2.54 (rSH/rAN) 1.91 (H/N) |
2.52 (rSH/rAN) 1.91 (H/N) |
17 (rSH/rAN) 16 (H/N) |
[69] | Metabolic—higher levels in cancer |
Those marked in bolditalic were chosen for western blotting
P* is not counted when protein is detected in only one group (100%). qvalue represents the statistical significance. The lower qvalue, the higher statistical significance. NA (not applicable) means that the protein was detected only in one group in a particular group comparison. If the protein was not a 0–1 protein, protein ratio is given. For example, there is 2.34 more amount of P14618 protein encoded by PKM gene in the hypoxia group but only when we compare it to the amount in the normoxia group
rAN: cells resistant to axitinib in normoxia, sAH: cells sensitized to axitinib in hypoxia, N: cells cultured in normoxia, H: cells cultured in hypoxia, rSH: cells resistant to sorafenib in hypoxia, sSN: cells sensitized to sorafenib in normoxia