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. 2017 Dec 13;5:e4109. doi: 10.7717/peerj.4109

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©2017 Salazar-Degracia et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Mean values and standard deviation of MyHC-I protein content in diaphragm (black bars) and gastrocnemius (grey bars) muscles as measured by optical densities in arbitrary units (OD, a.u.). Definition of abbreviations: MyHC, myosin heavy chain; F, formoterol. Statistical significance is represented as follow: * p ≤ 0.05 between non-cachexia controls and cancer-cachexia rats; §§§ p ≤ 0.001 between cancer-cachexia rats and cancer-cachexia rats treated with formoterol. (B) Mean values and standard deviation of MyHC-II protein content in diaphragm (black bars) and gastrocnemius (grey bars) muscles as measured by optical densities in arbitrary units (OD, a.u.). Definition of abbreviations: MyHC, myosin heavy chain; F, formoterol. Statistical significance is represented as follow: * p ≤ 0.05 between non-cachexia controls and cancer-cachexia rats; §§ p ≤ 0.01 and §§§ p ≤ 0.001 between cancer-cachexia rats and cancer-cachexia rats treated with formoterol. (C) Mean values and standard deviation of total MyHC protein content in diaphragm (black bars) and gastrocnemius (grey bars) muscles as measured by optical densities in arbitrary units (OD, a.u.). Total MyHC protein content was de sum of MyHC-I and MyHC-II protein content. Definition of abbreviations: MyHC, myosin heavy chain; F, formoterol. Statistical significance is represented as follow: * p ≤ 0.05 between non-cachexia controls and cancer-cachexia rats; § p ≤ 0.05 and §§§ p ≤ 0.001 between cancer-cachexia rats and cancer-cachexia rats treated with formoterol.