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Verification of rat RCECs. (A) Cells were cultured in endothelial cell medium for 10 days, and the characteristic cobble-stone morphology of RCECs was observed, scale bar: 100 μm, magnification: ×100. Immunocytochemical staining showed that the rat RCECs expressed vWf (B, green fluorescent dye) and CD31 (C, green fluorescent dye), scale bar: 40 μm, magnification: ×200. All nuclei were stained with DAPI (blue fluorescent dye).
Abbreviation: RCECs, retinal capillary endothelial cells.
