Table 4.

Schedule of biosample collection. The list indicates the planned use for a single microvial derived from each sample collected. Remaining microvials are banked, both at the local laboratories and at the central biorepository, for long-term storage and future testing

	Sample collected	Use of samples
Collected at baseline, 0 min, from all participants (fasting)	10 ml plain serum blood tube	Biochemistry, serology & insulin and C-peptide
	5 ml or 6 ml EDTA whole blood tubea	DNA extraction
	2 ml EDTA whole blood tube	Full blood count
	2 ml EDTA whole blood tube	HbA1c
	10 ml EDTA whole blood tube	Plasma extraction for viral genomics
	2 ml NaF blood tube	Fasting plasma glucose
	Spot urine collection	Albumin-to-creatinine ratio, urinary Na and K
Collected at 120 min (non-fasting) from survey participants only	4 ml plain serum blood tube	Insulin and C-peptide
	2 ml NaF blood tube	Plasma glucose

^aVolume of whole blood EDTA tube for DNA extraction will depend on local availability.