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. 2016 Jun 7;8(61):102852–102867. doi: 10.18632/oncotarget.9913

Figure 3. DRP1 interacts with CSA and is hyperactivated in CS-A cells.

Figure 3

A. Analysis of the interaction between CSA and DRP1 by TAP in the isogenic cell lines CS3BE and CS3BE-wtCSA after 20 µM CCCP treatment. B. Co-IP of DRP1 in the isogenic cell lines CS3BE and CS3BE-wtCSA. C. Analysis by western blotting of DRP1 and D. of its phosphorylated form at ser616 (p-DRP1) in the isogenic cell lines CS3BE and CS3BE-wtCSA after 20 µM CCCP treatment for different periods of time. Two independent experiments were performed with similar results. DRP1: 80kDa; HA-CSA: 45kDa; GAPDH: 37kDa.