Figure 2. Chemotherapeutic treatments affect activation of STAT3.

(A) Representative western blots showing the changes in pSTAT3 and STAT3 in MMTV-PyMT;Apc^Min/+ vs MMTV-PyMT;Apc^+/+ cells treated with cisplatin or doxorubicin for 24 hours. (B) Quantification of westerns blots shows that cisplatin nearly eliminates pSTAT3 in both cell lines. (C) Dual luciferase reporter assays measured STAT3 activation after treatment with cisplatin and doxorubicin and confirmed that cisplatin decreased STAT3 activity in both cells lines. (D) Representative western blots for Mcl-1 and Bcl-2 after 24-hour treatment with cisplatin and doxorubicin. (E) Quantification of western blots shows that chemotherapeutic agents do not affect Bcl-2 at the protein level. (F) Western blot quantification demonstrates that both cisplatin and doxorubicin decrease Mcl-1 in MMTV-PyMT;Apc^Min/+ cells. Each experiment was repeated 3 times and data are shown as means +/- SD; *P < 0.05 compared to solvent control, **P < 0.05 comparing MMTV-PyMT;Apc^Min/+ to MMTV-PyMT;Apc^+/+ cells