Figure 2. AKT is required for CD40 stimulation-mediated survival and inhibition of bendamustine-induced cell death.

CLL cells were cultured on a monolayer of control or CD154-expressing fibroblasts in the presence or absence of bendamustine at the indicated concentration with or without AZD5363 (10 μM) for 24 h (A) and 48 h (B). Co-cultured CLL cells were then harvested at the indicated time points and cell death was determined by PI inclusion on flow cytometry as described in Method. Each data set represents mean ± SD from 6 independent experiments using CLL cells from 4 different patients. Also actual data from individual CLL samples are shown in filled dot.