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. 2017 Nov 7;8(61):102948–102964. doi: 10.18632/oncotarget.22292

Figure 3. Stimulation by CD154 + IL-21 is more potent than that of CD154 + IL-4 in inducing proliferation of CLL cells in vitro.

Figure 3

(A) CFSE labelled CLL cells were co-cultured with CD154-expressing fibroblasts in the presence of recombinant human IL-4 (10 ng/ml) or IL-21 (12.5 ng/ml) over a period of up to ten days. CFSE labelled CLL cells co-cultured with parental fibroblasts were used as a negative control. At the indicated time points, co-cultured CLL cells were harvested and proliferation was measured by reduction of CFSE fluorescence intensity on flow cytometry as described in Method. Three representative CFSE histograms from the time-course experiments using primary CLL cells from 5 different samples are shown. (B) Pooled data analysis of the divided cell population among CLL cells co-cultured as in (A) shows that stimulation of CD40 + IL-21 induced significantly higher percentage of divided cells than that of CD40 + IL-4. Percentage of divided cells was calculated using FlowJo software as described in Method.