Skip to main content
. 2017 Nov 7;8(61):102948–102964. doi: 10.18632/oncotarget.22292

Figure 6. AKT is required for the induction of cyclin A2 and CDK1 by CD40 + IL-21 stimulation.

Figure 6

(A) CLL cells were cultured on a monolayer of control or CD154-expressing fibroblasts in the presence of recombinant human IL-21 (12.5 ng/ml) over a period of 3 days with or without 10 µM AZD5363. At the indicated time points, CLL cells were harvested and analysed for levels of cyclins A2, D2, D3 and E1, and p27 and p21 by Western blotting. β-actin was used as a loading control for densitometric analysis. One representative set of blots from the three of the 5 CLL samples described in Figure 5A is shown. (B) shows a pooled data analysis of the effect of AZD5363 on level of cyclin A2 in the co-cultured CLL cells as in (A). (C) CLL cells co-cultured under conditions as in (A) were harvested and analysed for levels of CDKs 1, 2 and 4 by Western blotting. β-actin was used as a loading control for densitometric analysis. One representative set of blots from the three of the 5 CLL samples described in Figure 5A is shown. (D) shows a pooled data analysis of the effect of AZD5363 on level of CDK1 in the co-cultured CLL cells as in (C).