Figure 9. The efficacy of inhibiting IRISOE TNBC cells secretome, in vivo.
(A) Schematic representative of experiments performed. (B) FACS analysis for CD29 and CD90 (MSC specific biomarkers), and (C) qRT-PCR analysis for CXCL1 mRNA expression in 3° IRISOE tumors developed in Nu/Nu mice treated with vehicle, Anakinra, SB265610, or both. Circulating CCL2 (D) or VEGF (E) levels in PB from naïve Nu/Nu mice or mice bearing 3° IRISOE orthotopic mammary tumor and treated for 4 days with vehicle, Anakinra, SB265610, or both.