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. 2017 Oct 17;8(61):103302–103314. doi: 10.18632/oncotarget.21871

Figure 3. NMuMG cells expressing TWIST have high levels of P-bodies.

Figure 3

(A) NMuMG cells lacking or expressing TWIST were treated without (Control) or with TGF-β (10 ng/ml) for 24 h, fixed and stained with anti-DCP1A to visualize P bodies (green). Nuclei were stained with DAPI (blue). (B) Average number of P-bodies per cell ± SEM (n > 150 cells per treatment) was quantified from triplicate experiments. *P < 0.001. (C) NMuMG cells expressing TWIST were treated without (Control) or with TGF-β or SB-431542 for 24 h, fixed and stained with anti-DCP1A to visualize P bodies (green). Nuclei were stained with DAPI (blue). Bar, 10 μm.