Figure 4. TGF-β promotes P-body accumulation HMLE and DG75 cells.

(A) HMLE cells were treated with TGF-β for the indicated times in days. Cells were fixed and stained with antibodies against DCP1A (green). Nuclei were stained with DAPI (blue). (B) HMLE cells were treated without (-) or with (+) TGF-β for 120 h. Cell lysates were examined by Western blotting using antibodies against E-cadherin, SYK, and GAPDH. (C) HMLE cells and HMLE cells expressing TWIST (TWIST) were fixed and stained with antibodies against DCP1A (green). Nuclei were stained with DAPI (blue). Bar, 10 μm.