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. 2017 Oct 9;8(61):103613–103625. doi: 10.18632/oncotarget.21665

Figure 5. Glycolysis and mitochondrial oxidative phosphorylation (OXPHOS) analysis of QGP-1 cells with and without knockdown of PCK2 and with or without treatment with RAD001.

Figure 5

(A) The change of extracellular acidification rate (ECAR) in of QGP-1/shLuc cells was not significantly changed by treatment with 50 nM of RAD001. The ECAR was slightly higher in QGP-1/shPCK2 (#1 and #2) cells than that in QGP-1/shLuc cells. The ECAR in QGP-1/shPCK2 cells was slightly reduced by RAD001 treatment. The lower figure shows the bar graph of ECAR in QGP-1/shLuc and GQP-1/shPCK2 cells with and without RAD001 treatment. (B) The oxygen consumption rate (OCR), including basal respiration, ATP production, and maximal respiration, in mitochondria were reduced in QGP-1/shPCK2 cells than that in QGP-1/shLuc cells. RAD001 induced a reduction in OCR, including basal respiration, ATP production but not maximal respiration, to QGP-1/shLuc and QGP-1/shPCK2 cells. The lower figure shows the bar graph of OCR in QGP-1/shLuc and QGP-1/shPCK2 cells with and without RAD001 treatment.