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. 2017 Oct 10;8(61):103640–103656. doi: 10.18632/oncotarget.21760

Figure 2. iPS-MSCs reduced apoptosis, potentiated proliferation and attenuated oxidative stress in albumin overloaded PTECs.

Figure 2

PTECs were treated with 10mg/ml human serum albumin (HSA) for 3 days in standard medium or medium supplemented with 20% iPSMSC-CM. TUNEL assay indicated that iPSMSC-CM reduced the number of apoptotic tubular cells upon albumin challenge. Representative graphs (A) and quantification (B) were presented. n=6 from three independent experiments. (C) MTT assay: cells were incubated with 10 mg/ml HSA for 1 day in standard medium or medium supplemented with 10%, 20% or 50% iPSMSC-CM. n=6 from three independent experiments. (D) Oxidative stress assay determined by absorbance of CM-H2DCFDA. Green signals indicate retention of CM-H2DCFDA in tubular cells. Original magnification ×400. (E) Pro-apoptotic and anti-apoptotic genes detected by real-time PCR. Data were obtained from three independent experiments. ***p<0.001 versus NIL or vehicle; #p<0.01, ##p<0.01 and ###p<0.001 versus HSA.