Skip to main content
. 2017 Oct 27;8(61):104206–104215. doi: 10.18632/oncotarget.22171

Figure 3. MiR-216b sensitizes NSCLC cells to cisplatin treatment through decreasing the expression of c-Jun.

Figure 3

(A) Western blot analysis was performed to evaluate the effect of c-Jun siRNA (50 pmol/ml) and c-Jun plasmid (2 μg/ml) on changing the cellular protein level of c-Jun in A549 and PC9 NSCLC cells. (B) MTT assay was performed to determine the viability of A549 and PC9 cells after they were treated with miR-216b mimics (50 pmol/ml), c-Jun plasmid (2 μg/ml) and cisplatin (2 μM). *P<0.05 vs. cisplatin + NCO group. #P<0.05 vs. cisplatin + miR-216b group. (C) After treatment with miR-216b mimics (50 pmol/ml), c-Jun plasmid (2 μg/ml) and cisplatin (2 μM), A549 and PC9 cells were stained with Annexin V followed by detection of cell apoptotic rate on flow cytometry. *P<0.05 vs. cisplatin + NCO group. #P<0.05 vs. cisplatin + miR-216b group. (D) Effect of c-Jun siRNA (50 pmol/ml) on cisplatin-induced (2 μM) cytotoxicity to A549 and PC9 NSCLC cells. *P<0.05 vs. cisplatin + NCO group.