Skip to main content
. 2017 Oct 31;8(61):104272–104285. doi: 10.18632/oncotarget.22210

Figure 3. Transfection, expression and establishment of bovine mammary epithelial stable cell lines with recombinant PiggyBac-LFcinB.

Figure 3

(A) Expression of Pmax GFP (positive control) after 24 h of transfection (100x); (B) Expression of recombinant bovine lactoferricin GFP after 24 h of transfection (100x); (C) Purified recombinant bovine lactoferricin cell line after 14 days of transfection in puromycin as selection marker (100x); (D) Purified recombinant bovine lactoferricin cell line after 14 days of transfection in puromycin which was used as selection marker at high resolution (200x). Cells showed the clear morphology of bovine mammary epithelial stem cells with nucleus and nucleolus.