Skip to main content
. 2017 Nov 1;8(61):104295–104302. doi: 10.18632/oncotarget.22223

Figure 2. MDA-MB468 cells increased in volume and then ruptured after NIR-PIT.

Figure 2

Representative images from 3D-LC QPM imaging depict morphological dynamics using three regimens of NIR light exposure time, 48 sec (A), 64 sec (B) and 76 sec (C). The cell in (A) swelled followed by bleb formation and then ruptured. The cell in (B) and the cell in (C) ruptured without antecedent bleb formation; it had already burst at the conclusion of the NIR light exposure. Red asterisk indicates a bleb. Yellow arrowhead indicates a flying fragment. (D) Comparison of cell volume before and immediately after NIR light exposure and maximum volume. Treated cells were all dramatically increased in volume. The cells significantly increased in cell volume compared to baseline after NIR-PIT. The cells exposed to longer NIR light, (e.g. 76 sec), didn’t show significant cell volume increases, which suggested that the damage was complete by the end of the light exposure. Data are means ± SE. n = 12 in 46 sec and 64 sec. n =10 in 76 sec. *P < 0.05, **P < 0.01, ***P < 0.005 versus the other group. n.s. indicates no significant difference. s indicates second or seconds.