Figure 8. Effect of piplartine-containing ruthenium complexes in the induction of apoptosis on HCT116 cells determined by flow cytometry using annexin V-FITC/PI staining after 24 and 48h of incubation.

The negative control (CTL) was treated with the vehicle (0.1% of a solution containing 70% sorbitol, 25% tween 80 and 5% water) used for diluting the compounds tested. Oxaliplatin (OXA, 3 μM) and piplartine (PL, 10 μM) were used as the positive controls. Data are presented as the mean ± S.E.M. of three independent experiments performed in duplicate. Ten thousand events were evaluated per experiment and cellular debris was omitted from the analysis. ^* p < 0.05 compared with the negative control by ANOVA followed by Student Newman-Keuls test.