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. 2017 Nov 6;8(61):104418–104429. doi: 10.18632/oncotarget.22301

Figure 4. In vivo tumorigenicity assay of tBMSCs, TERT-BMSCs and normal BMSCs.

Figure 4

(A) Representative image of a nude mouse transplanted with tBMSCs that produced subcutaneous tumors. All 10 nude mice transplanted with tBMSCs produce subcutaneous tumors in four weeks. (B) Live fluorescence imaging showing green fluorescence from the subcutaneous tumors derived from the tBMSCs. (C) Fluorescence imaging of a tumor section derived from the tBMSC transplanted nude mice. (D) H&E staining of tBMSC-derived tumor section shows disordered cell arrangement; tumor cells have big heterotypic nuclei with deep stained chromatin; cell proliferation is visible with rich blood vessels. (E-G) Gross morphology of tumors after subcutaneous implantation of TERT-BMSCs, tBMSCs, and BMSCs in nude mice. Note: subcutaneous implantation of BMSCs did not produce any visible tumor (G). (H) H&E stained TERT-BMSC-derived tumor tissue section showed tumor cells with big obvious heterotypic nuclei and deep stained chromatin.