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. Author manuscript; available in PMC: 2018 Dec 15.
Published in final edited form as: Cancer Res. 2017 Oct 16;77(24):6950–6962. doi: 10.1158/0008-5472.CAN-17-0981

Figure 2. NMT1 knockdown causes tumor suppression by down-regulation of Src kinase myristoylation and tyrosine phosphorylation.

Figure 2

(A) Correlation of Src and NMT1 expression (copy number from the TCGA database (n = 498 tumors). (B) PC-3 cancer cells infected with control shRNA or shRNA-Src were implanted subcutaneously in SCID mice (n=6 per group). The size and weight of xenograft tumors were measured at week 8. (C–D) PC-3 cancer cells were transduced with shRNA-NMT1 (MOI= 0, 10, 30, 50) by lentiviral infection. The transduced cells were grown with/without myristic acid-azide and myristoylated Src was detected by Click chemistry and immunoblotting with streptavidin-HRP (C). The expression levels of the indicated proteins from the protein lysates were measured by immunoblotting (D). (E) LNCaP, 22Rv1, and PC-3 cancer cells were transduced with control, shRNA-NMT1, shRNA-Src, or both and proliferation was measured by the MTT assay after 5 d. Data are relative to the control (set as 1). **: p<0.01 (each treatment group was compared with the control group). #: p<0.05; ##: p<0.01 (the compared groups were indicated in the figure).