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. 2017 Dec 12;8:1046. doi: 10.3389/fphys.2017.01046

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Copyright © 2017 Götting and Nikinmaa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparative gene expression levels between young and old erythrocytes (RBCs). Expression of eight target genes was normalized to β-actin as a reference gene and then related to the expression of the respective gene in the original erythrocyte sample taken before the fractionation procedure. Gene abbreviations are hbα-1, hemoglobin α-1; hbα-4, hemoglobin α-4; gbx, globin x1; β_3b-ar, β_3b-adrenergic receptor; β-nhe, β-Na⁺/H⁺-exchanger; hif1a, hypoxia inducible factor 1a; gapdh, glyceraldehyde-3-phosphate dehydrogenase; hsp70, inducible heat shock protein 70 kDa. Statistically significant differences between means of young and old erythrocyte fractions is shown by ^*p < 0.05.