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. 2017 Dec 12;8:1788. doi: 10.3389/fimmu.2017.01788

Figure 1.

Figure 1

Expression of model epitopes in wild-type and LdCen−/− parasites. (A) A immunoblot with α-Ld3′Nucleotidase antibody showing the native unmodified 3′Nucleotidase/nuclease and the chimera containing the 2W peptide in the lysates of the recombinant parasites. (B) An immunoblot with culture supernatants probed with α-Ld3′Nucleotidase antibody showing the secreted versions of the fusion protein 3′NT-2W. (C) An immunoblot with parasite lysates and culture supernatants probed with α-Ld3′nucleotidase antibody showing the secreted versions of the 3′NT-LLO fusion protein.