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. 2017 Dec 12;8:2473. doi: 10.3389/fmicb.2017.02473

FIGURE 1.

FIGURE 1

Type VI secretion system (T6SS) clusters on megaplasmid pBPHY01 of Paraburkholderia phymatum LMG 21445T. Nomenclature of the ORFs is according to the Burkholderia Genome Database (burkholderia.com). (A) T6SS-b cluster (position 510771–535444), structural elements coded between ORFs Bphy_5978 (BPHY_RS29885 – tssB) and Bphy_5997 (BPHY_RS29980 – tssA). (B) T6SS-3 cluster (position 654398–678783), structural elements coded between ORFs Bphy_6107 (BPHY_RS30485 – tssL) and Bphy_6124 (BPHY_RS30565 – tssI). The putative structural functions are marked in colors. Dashed lines indicate the beginning and the end of the putative operons based on preliminary RNA-seq data (Lardi et al., unpublished). Bphy_6125: (BPHY_RS38880) peptidase M23. Bphy_6127: PAAR repeat-containing protein. In bold are shown the genes mutated by insertion and the numbers indicate the position of the insertions (see “Materials and Methods”; Supplementary Table S1 and Table 1). Arrows indicate the external primers used for confirmation of the insertion (Bphy_5985For for the mutation in tssI-b gene and Bphy_6112Rev for the mutation in the tssB-3 gene – Supplementary Table S1).