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. 2017 Dec 12;8:2482. doi: 10.3389/fmicb.2017.02482

FIGURE 2.

FIGURE 2

Apis rhabdovirus-1 is detected in A. mellifera, B. impatiens, and V. destructor by RT-PCR. (A) Pools of nurse bees (BP) and mites (VP), respectively, from Israeli colonies 1 and 2 (B1P, V1P and B2P, and V2P, respectively, indicated above the figure). 1+2 (primers BRV-1-2683-F and BRV-1-4195-R) and 3+4 (primers BRV-1-10245-F and BRV-1-11804-R) in conserved regions used in the PCR reaction, see the section “Materials and Methods” and Table 1. M, GeneRuler Marker 1 kb DNA Ladder (Thermo Scientific Inc.); NTC, non-template control; Arrow, ARV-1. (B) Individual honey bees (B1, … , B10) and bumble bees (Bi1, … , Bi10) from U.S. samples. NTC, non-template control. Primers used 1+2 (BRV-1-2683-F and BRV-1-4195-R). Arrow, ARV-1.