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. 2017 Dec 12;8:2482. doi: 10.3389/fmicb.2017.02482

FIGURE 3.

FIGURE 3

Detection of the ARV-1 positive-sense RNA strand in V. destructor (V) and A. mellifera (B). The numbers indicate the individual tested. Suffix C indicates PCR control reaction from the same individual RNA performed on cDNA produced without any primer (see the section “Materials and Methods” and Table 1). PCR primers: BRV-1F-10356-R and TAG-D_F. M, GeneRuler Marker 1 kb DNA Ladder (Thermo Scientific Inc.); NTC, non-template control; Arrow, ARV-1 amplicon that was confirmed by sequencing (see the section “Materials and Methods”).