Table 1.
Primers and antibodies.
| Taqman expression assays | Gapdh | Mm99999915_g1 |
| Il1b | Mm00434228_m1 | |
| Il6 | Mm04207460_m1 | |
| Cxcl1 | Mm04207460_m1 | |
| Col1a1 | Mm00801666_g1 | |
| Fn1 | Mm01256744_m1 | |
| Serpinh1 | Mm00438058_g1 | |
| Acta2 | Mm00725412_s1 | |
| SYBR Green primer | Gapdh Pai-1 (Serpine1) |
Forward: ACAGTCCATGCCATCACTGC Reverse: GATCCACGACGGACACATTG Forward: GCCAGATTTATCATCAATGACTGGG Reverse: GGAGAGGTGCACATCTTTCTCAAAG |
| Antibody for Western Blot | Collagen type 1 | Primary: Rabbit anti-collagen type 1 (1:2,000; Rockland Immunochemicals, Limerick, USA) Secondary: Goat anti-rabbit HRP (1:2,000, Dako, Santa Clara, USA) |
| α-smooth muscle actin (α-SMA) | Mouse anti-α-SMA clone 1A4 (1:5,000; Sigma-Aldrich, St. Louis, USA) Secondary: Rat anti-mouse HRP (1:5,000, Dako) | |
| Heat-shock protein (HSP47) | Rabbit anti-HSP47 (1:2,000; ab109117 Abcam, Cambridge, USA) Secondary: Goat anti-rabbit HRP (1:2,000, Dako) |