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. 2017 Dec 12;10:413. doi: 10.3389/fnmol.2017.00413

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Copyright © 2017 Greenwood, Greenwood, Gillard, Chitra Devi and Murphy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Upregulation of cAMP responsive element binding protein 3-like 1 (Creb3l1) expression by cyclic AMP (cAMP) pathway. (A) Immunofluorescent staining in paraventricular nucleus (PVN) and supraoptic nucleus (SON) of euhydrated and 3 days dehydrated rat. (B) Immunoblot analysis of total protein extracts from PVN and SON of euhydrated, 3 days dehydrated and 7 days salt loaded rats. (C) qRT-PCR analysis of Creb3l1 in PVN and SON of euhydrated and 3 days dehydrated rat. (D) Expression of full-length and cleaved Creb3l1 protein in response to forskolin (FSK) treatment. (E) Detection of full-length and cleaved Creb3l1 protein forms in AtT20 cells 48 h after transfection with Flag-Creb3l1 construct. Densitometry analysis was performed by using Quantity One imaging software (Bio-Rad). (F) Upregulation of Creb3l1 mRNA by foskolin in AtT20 cells determined by qRT-PCR. (G) Immunoblot showing Creb3l1 cleavage products in AtT20 cells overexpressing N-terminal Flag tagged Creb3l1 construct. In (B) *indicates the detection of non-specific bands by the Creb3l1 antibody in brain samples. DH, dehydration; SL, salt loading; M, molecular weight marker. Values are means + SEM of n = 3−4 per group. **p < 0.01, ***p < 0.001. Scale bars = 100 μm.