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. 2017 Dec 12;10:413. doi: 10.3389/fnmol.2017.00413

Figure 2.

Figure 2

Regulation of Creb3l1 expression. (A) AtT20 cells were pretreated with signaling pathway inhibitors for 24 h before treatment with vehicle (DMSO) or stimulation with FSK (10 μM) for 6 h. The expression of Creb3l1 was evaluated by qRT-PCR. (B) AtT20 cells were pretreated with CHX or vehicle for 2 h followed by FSK addition to culture media for up to 8 h and Creb3l1 mRNA expression was investigated by qRT-PCR. (C) Transcription factors mined from microarray data that increase by greater than two-fold in the SON of the dehydrated mouse (2 days) and rat (3 days) compared to euhydrated controls. (D) Relative mRNA expression was investigated by qRT-PCR in PVN and SON of euhydrated, dehydrated (3 days) and salt loaded (7 days) rats and after a single intraperitoneal injection of 1.5 ml/100 g body weight 1.5 M NaCl solution over a 4 h experimental period compared to controls. DH, dehydration; SL, salt loading; CHX, cycloheximide. Values are means + SEM of n = 5–6 per group (animal) n = 3 per group (cell). ##p < 0.01 (t-test). **p < 0.01, ***p < 0.001.