Skip to main content
. 2017 Dec 12;10:413. doi: 10.3389/fnmol.2017.00413

Figure 4.

Figure 4

Identification of Nr4a1 as a novel regulator of the Creb3l1 gene. (A) Schematic representation of potential Nr4a1 responsive elements in the rat Creb3l1 promoter region. (B) Sequences of NurRE and NBRE sites in the rat Creb3l1 promoter. (C) Rat Creb3l1 promoter-luciferase reporter constructs with chemical treatments (4 h) in AtT20 and N2a cells. (D,E) Luciferase assays using plasmids containing deletion constructs of the Creb3l1 promoter-luciferase reporter together with overexpression of Nr4a1 or 4 h FSK treatment. (E) Restriction enzyme generated deletion mutants of the Creb3l1 promoter to pinpoint the interacting site of Nr4a1 with the promoter. (F) Luciferase assays using Nr4a1 responsive element site-directed deletion constructs of the Creb3l1 promoter with overexpression of Nr4a1 or 4 h FSK treatment. Veh, vehicle; FSK, forskolin; DH, dehydration. Values are means + SEM of n = 3−4 per group. *p < 0.05, **p < 0.01, ***p < 0.001.