Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 12;10:413. doi: 10.3389/fnmol.2017.00413

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Greenwood, Greenwood, Gillard, Chitra Devi and Murphy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Nr4a1 regulates Creb3l1 transcription by binding at the promoter. (A) Validation of HA tagged Nr4a1 construct by immunoblot in AtT20 cells and immunofluorescence in AtT20 and N2a cells. (B) ChIP assays were performed using antibody against HA tag in Nr4a1 and HA-Nr4a1 transfected cells. Two sets of primers (arrow heads) were designed to amplify promoter regions close to NBRE2 and NBRE3 consensus sequences by RT-PCR. PCR products from the ChIP assay were verified by gel electrophoresis. Scale bars = 10 μm.