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. 2017 Dec 12;10:413. doi: 10.3389/fnmol.2017.00413

Figure 7.

Figure 7

Transcriptional regulation of Creb3l1 expression in the rat PVN. (A) Fluorescent in situ hybridization marking the distribution of Avp and Ot mRNAs in the SON and PVN. The toluidine blue stained section illustrates the punches collected for mRNA analysis. In the PVN the enrichment of AVP positive neurones in the posterior magnocellular bundle (labeled AVP) and OT positive neurones more medially (labeled OT) enabled a level of separation of these subdivisions. A 27 gauge needle was then used to create small tracts lateral to the posterior magnocellular subdivision of the PVN (labeled marker) for orientation of punching. (B) qRT-PCR analysis of Avp and Ot RNAs in AVP and OT punches in the euhydrated and 3 days dehydrated rat PVN. (C) qRT-PCR analysis of Nr4a1 and Creb3l1 expression in AVP and OT punches in the euhydrated and 3 days dehydrated rat PVN. (D) Methylation status of 12 CpGs on the Creb3l1 promoter in DNA extracted from AVP and OT punch samples of euhydrated and dehydrated rat. DH, dehydration. Values are means + SEM of n = 4 per group. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars = 100 μm.