Table 2.
Kinetic parameters of recombinant OsCCR19, 20 and 21 catalyzed reaction with hydroxycinnamoyl-CoAsa.
| OsCCR | Substrate | KM (μM) | Vmax (μmol min−1mg−1) | kcat (min−1) | kcat/KM (μM−1 min−1) |
|---|---|---|---|---|---|
| OsCCR19 | p-Coumaroyl-CoA | 36.66 ± 3.06 | 0.51 ± 0.11 | 21.17 | 0.60 |
| Feruloyl-CoA | 26.85 ± 6.68 | 0.28 ± 0.12 | 11.48 | 0.43 | |
| Sinapoyl-CoA | 62.54 ± 14.08 | 0.83 ± 0.04 | 34.19 | 0.55 | |
| OsCCR20 | p-Coumaroyl-CoA | 24.08 ± 3.05 | 0.17 ± 0.02 | 7.72 | 0.32 |
| Feruloyl-CoA | 15.71 ± 1.55 | 0.58 ± 0.12 | 22.61 | 1.41 | |
| Sinapoyl-CoA | 23.34 ± 2.60 | 0.13 ± 0.01 | 5.64 | 0.24 | |
| OsCCR21 | p-Coumaroyl-CoA | 16.36 ± 4.69 | 0.02 ± 0.003 | 1.12 | 0.08 |
| Feruloyl-CoA | 2.70 ± 1.69 | 0.03 ± 0.003 | 1.59 | 0.77 | |
| Sinapoyl-CoA | 10.20 ± 1.84 | 0.02 ± 0.004 | 0.72 | 0.07 |
a
The results represent the mean ± standard deviation of four independent experiments.