Figure 3.

Mdivi-1 protects against cisplatin-induced hair cell death. (A) Concentrations of mitochondrial division inhibitor mdivi-1 between 1–5 μM are well tolerated by zebrafish; whereas 10 μM of mdivi-1 is toxic to hair cells (n = 5 larvae per data point, from three clutches). (B) Application of 3 or 7 μM of mdivi-1 allowed significantly more hair cells to survive treatment with 50 and 100 μM of cisplatin. (C) Representative maximum-intensity projection images of pvalb3b::GFP neuromast hair cells treated with 50-μM-cisplatin and/or 50-μM-mdivi-1 (middle and right images). Data are mean, error bars indicate SEM. **p < 0.001 and *p < 0.05, in comparison to no mdivi-1 treatment within the same cisplatin concentration (see Supplementary Table S1). Scale bar: 10 μm.