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. 2017 Dec 12;8:2049. doi: 10.3389/fpls.2017.02049

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Copyright © 2017 Kakrana, Kumar, Satheesh, Abdin, Subramaniam, Bhattacharya, Srinivasan, Sirohi and Jain.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis of nematode-responsive root-specific genes using Quantitative Real time PCR. Relative expression of six NRRS genes (At1g48670, At5g58780, At3g29775, At1g80320, At1g74770, and At2g18140) along with At5g26530, a nematode-responsive root-specific gene used as positive control (Kumar et al. 2016) in control and nematode infected plant root samples at 10- and 21- dpi. The transcripts levels were normalized to the expression of a plant UBQ10 gene. The data are shown as −ΔΔC_t and each bar represents the mean ± SE (n = 3). An asterisk indicates statistical significance difference in a one-way ANOVA and Tukey test (p ≤ 0.05).