Figure 5.

The number, size and area fraction of Rab5a-immunoreactive early endosomes are increased in the cingulate cortex of APOE4/4 vs. APOE3/3 mice with age. Rab5a-immunoreactive early endosomes were quantified in the cingulate cortex of 6, 12, 18, and 25 month-old APOE3/3 and APOE4/4 mice. (A) Representative images showing Rab5a immunolabeling (red). Black and white images were generated in ImageJ and used for quantification. (B–D) Quantification of average Rab5a-positive early endosome number (B), size (C), and area fraction (D) in APOE4/4 vs. APOE3/3 mice. (^***p < 0.001; findings are normalized to the mean APOE3/3 value at each age in order to compare between age groups).