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. 2017 Dec 12;8:904. doi: 10.3389/fphar.2017.00904

FIGURE 2.

FIGURE 2

Effects of GHK treatment on BLM-induced collagen deposition and assessments of fibrosis severity in mice. (A) Lung sections were stained with MT and then numerically scored for fibrosis severity and distribution. Representative photomicrographs showing the severity scores of the different groups are shown. (B) The fibrosis index was calculated with the severity score as follows: (0 ∼ 4) × % area affected (0 ∼ 100%)/100. (C) The lung sections were graded by two independent pathologists blinded to the treatment groups. The mean calculation of the difference of fibrosis severity index by the two individual pathologists has been shown. (D) Total collagen in lung tissue was detected by a Sirius Red Total Collagen Detection Kit. BLM increased collagen deposition in lungs of mice, whereas GHK treatment decreased collagen deposition in lung tissues. The bars represent the mean ± SEM values; statistical analysis was performed by one-way ANOVA and Turkey’s multiple-comparison test; compared with control group, ##P < 0.01; compared with BLM group, ∗P < 0.05, ∗∗P < 0.01.