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. 2017 Dec 12;8:1710. doi: 10.3389/fimmu.2017.01710

Figure 1.

Figure 1

Invariant natural killer T (iNKT) cells are recruited to the site of inflammation induced by monosodium urate (MSU) crystal. (A) Outline of the synovium-like mouse subcutaneous air-pouch model. Subcutaneous air pouches were generated by injection of 5 mL air into the subcutaneous tissue of the back, followed by injection of another 3 mL of air at day 3 and day 5. At day 7, MSU crystal (3 mg in 1 mL) was injected into air-pouch cavities. (B) Flow cytometry analysis identified iNKT cells (TCRβhi and CD1d-tetramerhi) in wild-type (WT) air-pouch cavities at 12 h post-MSU crystal challenge (right panel); the bar graph shows the absolute number of iNKT cells infiltrating the air-pouch cavities at different time points (left panel). Results are representative of three independent experiments. Values are the mean and SEM (n = 3–4 per group). Significance for all data was determined by unpaired Student’s t-test: ns, not significant; *P ≤ 0.05; **P ≤ 0.01.