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. 2017 Dec 12;8:1048. doi: 10.3389/fphys.2017.01048

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Copyright © 2017 Buttler, Jordão, Fragas, Ruggeri, Ceroni and Michelini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Combined effect of aerobic training (T) and angiotensin II (Ang II) infusion on microglia expression (IBA1 ir) in autonomic nuclei of spontaneously hypertensive rats (SHR) aged 3 months. (A) Representative images comparing IBA1 expression (blue) within the paraventricular nucleus of the hypothalamus (PVN) in SHR-T infused with saline or angiotensin II (Ang II). Insets depict high magnification of areas delimited by the white rectangle and show both the maintenance of inactivated microglia in SHR-T+Saline and activated pattern in SHR-T+Ang II. (B) Quantification of IBA1 density in the PVN, nucleus of tract solitary (NTS) and rostral ventrolateral medulla (RVLM) in SHR-T infused with saline or Ang II icv. IBA1 expression in the sedentary (S) SHR infused with saline is also shown for comparison. Measurements were made in 7–9 slices, 3–4 rats/group. Significances (P < 0.05) are ^†vs. S+saline; ^*vs. T+ saline.