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. Author manuscript; available in PMC: 2018 Apr 7.
Published in final edited form as: J Proteome Res. 2017 Mar 27;16(4):1579–1592. doi: 10.1021/acs.jproteome.6b00972

Figure 2.

Figure 2

RT-PCR analysis of uncharacterized proteins during differentiation of human airway epithelial cells in vitro. RNA was isolated from air/liquid interface cultures on the indicated days and specific primers for each of the proteins were used to analyze relative expression level. Expression of all of the uncharacterized proteins increased during differentiation. The known ciliary component, dynein axonemal intermediate chain 1 (DNAI1) was amplified as a positive control, while cyclophilin A (PPIA) was amplified as a loading control; reactions with no RNA (H2O) were used as a negative control.