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Western blot showing the expression of the uncharacterized proteins in whole cell lysates from undifferentiated (3 days) and differentiated (greater than 4 weeks) HBE cells and isolated ciliary axonemes. All proteins were undetectable in the undifferentiated cells but were clearly present in the isolated axonemes. Samples were also probed for the known ciliary component DNAI1 as a positive control, while CALRETICULIN was used as a loading control.
