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. Author manuscript; available in PMC: 2018 Apr 7.
Published in final edited form as: J Proteome Res. 2017 Mar 27;16(4):1579–1592. doi: 10.1021/acs.jproteome.6b00972

Figure 5.

Figure 5

a) Localization of CCDC181 (red) and ezrin (green) confirms that CCDC181 is a component of the ciliary axoneme and not the microvilli. A low magnification bright field image of the stained ciliated cells (a) imaged with super resolution microscopy (b) showing that CCDC181 localizes specifically to the cilia (scale bar = 2.5 µm). c) Co-localization of Ac-TUB (blue), ERICH3 (green), and CCDC181 (red) by immunofluorescence localizes CCDC181 to the proximal portion of airway cilia. Cell nuclei were stained with Hoechst 33342 (gray), scale bar = 5 µm.