Table 1.
Tubulin isoforms identified by LC/MSE in ciliary axonemes. The number of unique peptides from each isoform is shown.
| Accession Number | Alpha tubulin | Number of Exclusive Peptides |
|---|---|---|
| * TBA8_HUMAN | Tubulin alpha-8 chain OS=Homo sapiens GN=TUBA8 PE=1 SV=1 | 7 |
| TBA4A_HUMAN | Tubulin alpha-4A chain OS=Homo sapiens GN=TUBA4A PE=1 SV=1 | 6 |
| TBA4B_HUMAN | Putative tubulin-like protein alpha-4B OS=Homo sapiens GN=TUBA4B PE=5 SV=2 | 5 |
| * TBA3E_HUMAN | Tubulin alpha-3E chain OS=Homo sapiens GN=TUBA3E PE=1 SV=2 | 2 |
| * TBAL3_HUMAN | Tubulin alpha chain-like 3 OS=Homo sapiens GN=TUBAL3 PE=1 SV=2 | 2 |
| * TBA3C_HUMAN | Tubulin alpha-3C/D chain OS=Homo sapiens GN=TUBA3C PE=1 SV=3 | 1 |
| * TBA1C_HUMAN | Tubulin alpha-1C chain OS=Homo sapiens GN=TUBA1C PE=1 SV=1 | 1 |
| Beta Tubulin | ||
|
| ||
| TBB8_HUMAN | Tubulin beta-8 chain OS=Homo sapiens GN=TUBB8 PE=1 SV=2 | 8 |
| TBB6_HUMAN | Tubulin beta-6 chain OS=Homo sapiens GN=TUBB6 PE=1 SV=1 | 8 |
| TBB1_HUMAN | Tubulin beta-1 chain OS=Homo sapiens GN=TUBB1 PE=1 SV=1 | 8 |
| TBB5_HUMAN | Tubulin beta chain OS=Homo sapiens GN=TUBB PE=1 SV=2 | 7 |
| TBB3_HUMAN | Tubulin beta-3 chain OS=Homo sapiens GN=TUBB3 PE=1 SV=2 | 5 |
| TBB8L_HUMAN | Tubulin beta-8 chain-like protein LOC260334 OS=Homo sapiens PE=1 SV=1 | 4 |
| * YI016_HUMAN | Putative tubulin beta chain-like protein ENSP00000290377 OS=Homo sapiens PE=5 SV=2 | 3 |
| TBB4Q_HUMAN | Putative tubulin beta 4q chain OS Homo sapiens GN TUBB4Q PE 5 SV 1 | 3 |
| TBB4B_HUMAN | Tubulin beta-4B chain OS=Homo sapiens GN=TUBB4B PE=1 SV=1 | 2 |
| TBB4A_HUMAN | Tubulin beta-4A chain OS=Homo sapiens GN=TUBB4A PE=1 SV=2 | 2 |
| * TBB2A_HUMAN | Tubulin beta-2A chain OS=Homo sapiens GN=TUBB2A PE=1 SV=1 | 2 |
| TBB2B_HUMAN | Tubulin beta-2B chain OS=Homo sapiens GN=TUBB2B PE=1 SV=1 | 1 |
| * TBB4_HUMAN | Tubulin beta 4 chain OS Homo sapiens GN TUBB4 PE 1 SV 2 | 1 |
*
These particular isoforms did meet the stringency threshold for inclusion in the full proteomic dataset (95% Protein Probability, 80% Peptide Probability, 2 Peptide Minimum). They were nonetheless included in this list of isoforms due to the evidence for their presence in the axoneme. The complete list of tubulin peptides recovered is shown in Table SI-4.