Table 3.
Relative molar amount of radial spoke components determined from 3 separate axonemal isolates. The amount of each protein was determined by LC/MSE and normalized to the radial spoke protein with the lowest abundance: the average relative amount is shown (see also supplemental figure S#. The estimated stoichiometry is compared to the estimated stoichiometry of Chlamydomomas, as reported in Pigino et al., 2011 (57).
| Identified Proteins | Accession Number |
Molecular Weight |
Average Relative Amount |
Estimated Stoichiometry |
Chlamydomonas reinhardtii |
Estimated Stoichiometry$ |
|---|---|---|---|---|---|---|
| Radial spoke head protein 4 homolog A | RSH4A_HUMAN | 81 kDa | 11.6 | 12 | RSPH4A | 4 |
| Radial spoke head protein 9 homolog | RSPH9_HUMAN | 31 kDa | 7.8 | 8 | RSPH9 | 4 |
| Radial spoke head 1 homolog | RSPH1_HUMAN | 35 kDa | 5.8 | 6 | RSPH1 | 4 |
| DnaJ (Hsp40) related, subfamily B, member 13 | B3LEP4_HUMAN | 36 kDa | 5.2 | 5 | RSP16 | 2 |
| Radial spoke head protein 3 homolog | RSPH3_HUMAN | 64 kDa | 4.2 | 4 | RSPH3 | 4 |
| Ropporin-1-like protein | ROP1L_HUMAN | 26 kDa | 3.5 | 4 | RSP11 | 2 |
| Rhabdoid tumor deletion region protein 1 | RTDR1_HUMAN | 39 kDa | 2.1 | 2 | RSP14 | 1 |
| Nucleoside diphosphate kinase homolog 5 | NDK5_HUMAN | 24 kDa | 1.9 | 2 | RSP23 | 2 |
| Peptidyl-prolyl cis-trans isomerase-like 6 | PPIL6_HUMAN | 35 kDa | 1.6 | 2 | RSP12 | 2 |