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Radioactivity distribution within gradient fractions of CuFi-1 cells infected or not with P. aeruginosa. Cell sphingolipids were labeled at the steady state with [1-3H]-sphingosine. We isolated the lipid rafts from infected and noninfected cells as the detergent-resistant membrane (DRM) following the procedure described in details in “Materials and Methods”. After ultracentrifugation, we collected eleven fractions and we measured the radioactivity associated to each of them.
