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. 2017 Dec 3;2017:1730245. doi: 10.1155/2017/1730245

Figure 10.

Figure 10

Radioactivity distribution within gradient fractions of CuFi-1 cells infected or not with P. aeruginosa. Cell sphingolipids were labeled at the steady state with [1-3H]-sphingosine. We isolated the lipid rafts from infected and noninfected cells as the detergent-resistant membrane (DRM) following the procedure described in details in “Materials and Methods”. After ultracentrifugation, we collected eleven fractions and we measured the radioactivity associated to each of them.